FastQCFastQC Report
Mon 27 Feb 2023
SRR3128312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488365
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311981.2537549756567063No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC58670.23577730758952165No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG54990.22098848038772448No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG47390.19044633725357815No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41470.16665561523329578No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTAAGTCCT40180.16147148830657881No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA36770.14776771092665264No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27960.11236293710930673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95450.084.374781
CGTAGGG6100.075.502612
TCCGTAC23650.075.114952
CCGTACA23200.074.546213
TAGGGTA9150.073.961754
ATAGCGG5100.073.791211
AGTAGGG39450.072.549762
GTAGGGT10900.072.435013
ATCCGTA24700.072.372151
CGTACAC23900.071.9695744
TAGGGCA12800.071.963434
AGGGATG35600.071.682915
GGTAAGG13950.070.478541
AGGGCAT21500.069.948315
AAGGGAT39950.069.759544
AGGGTAC11100.069.436315
AAGAGGG84400.069.269762
ATAGGGA20650.068.958583
GTAGGGC9100.068.6872253
ATGTAGG8150.068.109741