FastQCFastQC Report
Mon 27 Feb 2023
SRR3128310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422082
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327421.3518122012384388No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC52920.2184897125695992No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG50960.2103975009929474No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG45190.18657502099433462No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41990.17336324699163777No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28110.11605717725494016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102600.084.457571
AGTAGGG36450.073.331942
GTAGGGA17500.072.874173
GGTAAGG13700.072.836231
AGGGCAT21000.072.740645
AGGGATG37200.072.650385
TAAGGGA21350.071.635433
TAGGGCA13300.071.4730154
AAGGGAT37450.071.12254
ATAGCGG4700.071.103771
AGTAAGG12200.070.603041
GAGGGAT32500.070.081664
AGGGAAT24750.069.315555
CGTAGGG5650.069.130872
ATAGGGA21950.068.819723
TAGGGTA7750.068.6150054
TAACGGG8050.067.8116762
TAGACGG3550.067.619811
TAGGGAT15700.067.441194
TAGAGGG43600.067.2425162
Produced by FastQC (version 0.11.5)