FastQCFastQC Report
Mon 27 Feb 2023
SRR3128309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593869
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196091.230276766785727No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACCATAGA66180.41521605602467954No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG55600.3488366986245419No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC50730.3182821172881837Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG47610.2987071083006194No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA32380.2031534586594005No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29220.18332748801814955No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATG20570.12905703040839617No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19130.12002241087567422No Hit
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT16470.1033334609055073No Hit
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG16170.10145124850285689No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65900.087.168671
CCGTACA29300.078.595043
CGTACAC29350.077.9807744
CGGGTAT1900.076.678715
AGGGTAA7200.075.716795
TCCGTAC32200.075.457322
GAATAGG9250.075.2748951
AGGGTAC6650.074.911925
TAGGGTA5600.074.691064
AGGGATG21000.074.299435
ATCCGTA33100.074.052521
GAGGGAT20250.072.873794
CGTAGGG4200.072.732882
GGTAAGG9350.072.4571151
TAGGGCA8550.071.4568564
AGGGCAT12600.070.8679355
AGGGATC12500.069.555015
ATAGGGA14200.069.502053
AGTAGGG24850.069.407492
AGATAGG8950.069.3874741
Produced by FastQC (version 0.11.5)