FastQCFastQC Report
Mon 27 Feb 2023
SRR3128307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531676
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205761.3433650458713202No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG50600.3303570729057581No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC44070.2877240356315565Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG41530.2711408940271963No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28070.18326330111590178No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACCATAGA20750.13547251507498975No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19420.12678921651837596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67900.086.187581
CGTAGGG4050.077.856112
AGGGTAC6800.077.413115
AGGGCAT11550.077.317365
GAATAGG8500.075.883081
TAGGGTA5750.073.66274
GAGGGAT19350.072.478334
AGGGATG23850.072.127045
AGTACGG2700.071.492981
TAGGGCA7900.070.295554
TAGAGGG26800.070.24222
ATAGCGG4250.069.7902761
ATAGGGC9650.069.739953
AGTAGGG24700.069.7362
AAGGGTA9650.069.252264
GTAGGGA9750.069.024673
TAGCGGG8000.068.8285752
TAAGGGA13100.068.6175163
AGTAAGG8550.068.2808461
CGGGTAT1800.067.890075
Produced by FastQC (version 0.11.5)