FastQCFastQC Report
Mon 27 Feb 2023
SRR3128306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072434
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228281.1015067307330415No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG54650.2636995918808512No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATTATGC54100.26104570760757645No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC51990.2508644424864676Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG51600.24898259727450908No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA40380.19484335809970305No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26890.12975081474247188No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23380.1128142078348454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82450.082.575081
TAGGGCA10350.076.733114
CCGTACA23500.076.38923
TCCGTAC24250.075.964512
CGTAGGG6350.075.485382
CGTACAC24300.073.680944
ATCCGTA25800.072.218191
AGTAAGG13700.071.092031
ATAGGGC13900.070.9971543
AGGGATG27600.069.468445
ATAGAGG15650.069.1487961
TAAGGGA14350.069.098243
AGGGAAT19300.068.907365
AGTAGGG32500.068.5382
GAGGGAT24050.068.3894964
AGGGCAT17650.068.160335
TAGGGTA6000.068.1403664
TAGCGGG10500.068.028472
ATAAGGG24950.067.240992
AGGGTAC9300.066.700245
Produced by FastQC (version 0.11.5)