FastQCFastQC Report
Mon 27 Feb 2023
SRR3128305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303796
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273291.186259547286305No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG63220.27441665841940865No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC60550.2628270905930907Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG54850.23808531658185014No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATTATGC31360.13612316368289554No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29450.1278324990580763No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27850.12088743968650002No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26380.1145066663888643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92700.080.770151
CGTAGGG7000.075.206082
TAGGGCA11000.074.3498464
AGTAAGG13300.073.199191
TAGCGGG12100.071.476852
AGGGATG30950.071.073675
ATAGAGG17100.070.684561
AGGGCAT20100.070.3872455
AGATAGG14100.070.0467151
AGGGTAC11600.069.288585
ATAGCGG5600.068.8673551
AGTAGGG37000.068.346062
ATAGGGC14100.068.338863
TATAGGG17100.067.894382
TAAGGGA17050.067.266433
GATAGGG35700.067.1482852
TAGGGTC7300.066.962874
AATGCGG4950.066.5089951
CGAAGGG23500.066.405372
TAGAGGG41800.066.345072
Produced by FastQC (version 0.11.5)