FastQCFastQC Report
Mon 27 Feb 2023
SRR3128304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018213
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238431.1813916568766527No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG50490.2501718104085148No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG46570.230748687081096No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC46400.2299063577531212Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27030.1339303631479928No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22730.11262438602863027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84950.081.9009251
TAGGGCA11250.078.221614
GGTAAGG11900.074.368441
ATAGGGC14400.073.528843
AGGGATG29800.073.177835
AGTAAGG14300.072.750171
AGGGCAT19100.070.1276555
TAGGGTC6450.069.3109054
GAATAGG12700.068.942491
GAGGGAT26800.068.831774
GATAGGG33550.068.588932
AGTAGGG31400.068.339672
TAGCGGG8550.068.248522
ATAGCGG3550.067.6269151
AGGGTAC7600.067.4047855
AAGGGCA33000.067.307864
TAGAGGG33550.067.186292
AAGAGGG68650.066.286282
TAACGGG6400.066.175962
CGAAGGG18150.065.855922
Produced by FastQC (version 0.11.5)