FastQCFastQC Report
Mon 27 Feb 2023
SRR3128303.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128303.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340138
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308561.3185547177132289No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAGATGGC33810.14447865895088238No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG31080.13281268027782978No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG30250.12926588090104088No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29910.12781297513223577No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC29750.12712925477044518Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24770.10584845850971182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104500.085.059571
AGTAGGG41050.074.1863252
CCGTACA16500.073.484853
CGTACAC16850.071.4006354
TCCGTAC17450.071.369472
AGTAAGG16800.070.2654951
ATCCGTA17800.070.0170061
CGTAGGG5950.069.506872
ATAGGGA20050.069.146413
TAGGGCA13050.068.423474
ATAGGGC13850.068.203763
TAAGGGA19850.067.475553
AGGGATG33900.067.097715
GTACGGG5900.066.909742
GAATAGG13700.066.597631
ACGGGTA2350.065.994554
AAGGGTA15050.065.8882454
GTAGGGA19200.065.843523
TAGCGGG10500.065.3469852
AGGGTAC11250.065.167955
Produced by FastQC (version 0.11.5)