FastQCFastQC Report
Mon 27 Feb 2023
SRR3128302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609392
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365941.4023956538534645No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG34840.13351769301047908No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG34310.13148656851864343No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC34080.13060513713539398Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28170.10795618289624556No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26340.10094305493386965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116500.085.6786961
AGTAGGG42300.073.3399352
AGTAAGG17750.071.286511
TAGGGCA15300.071.271674
TAAGGGA20800.070.732333
ATAGGGA22650.069.935663
CGTAGGG8450.069.53292
TAGGGTA10100.068.8748554
TAGGGAT17450.068.416154
TAGCGGG13950.067.726532
GTAGGGA22500.067.6861043
GAATAGG14200.067.245171
AGGGATG40250.066.7965
ACGGGTA3100.066.7131354
GTAGGGT11300.066.554673
GTAGGGC11800.066.522943
TAGAGGG46000.066.419032
ATAGCGG4750.066.349031
AAGGGTA18700.066.105034
AGGGTAC13250.065.9807745
Produced by FastQC (version 0.11.5)