FastQCFastQC Report
Mon 27 Feb 2023
SRR3128301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272635
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319881.4075291456833148No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG28790.12668114325441612No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC27890.12272098247188837Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG27570.12131292530476738No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25500.11220455550495351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108350.084.80061
ATAGCGG4850.074.768651
ATAGGGC14250.074.6557163
TAGGGCA11650.072.730424
AGTAGGG38650.070.762922
GTAGGGT11400.070.6091163
ACGGGTA2950.070.21024
TAGCGGG12900.070.063312
ATAGGGA19700.070.011753
AGGGCAT18450.068.7808465
TAGGGTA8800.068.4694444
TAAGGGA19300.067.804273
GTAGGGA17850.067.510493
TAGAGGG40100.067.382422
AGGGTAC11800.067.31395
AGTAAGG16250.066.94671
AGGGATG34450.066.850855
GAGGGAT31400.066.711374
TATAGGG16150.066.165632
GTACGGG6000.065.903292
Produced by FastQC (version 0.11.5)