FastQCFastQC Report
Mon 27 Feb 2023
SRR3128300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450574
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291651.1901293329644402No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC45550.18587481953207696No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTCACCCG45400.18526271804075292No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG41420.16902162513762084No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38000.15506571113543194No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG37870.15453522317628443No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA31680.1292758349676443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94450.084.431041
TAGGGCA11300.077.355314
CCGTACA20600.073.6870043
TCCGTAC21850.072.697742
AGTAGGG38900.072.244912
ATCCGTA22250.071.6811
CGTACAC21200.071.379854
ATAGGGA19050.070.554823
ACGGGAT7900.070.19574
ATAGGGC12800.069.758843
AGGGTAC10450.068.806715
ATGCGGG11150.068.701842
GTAGGGC9800.068.574973
GAATAGG13400.068.46431
TAGGGTA8350.068.101164
GTAGGGA15850.067.8987663
GGTAAGG14350.067.866091
TATAGGG19750.067.340292
ATAGAGG17050.067.3286361
ATGAGGG37850.066.9234852
Produced by FastQC (version 0.11.5)