FastQCFastQC Report
Mon 27 Feb 2023
SRR3128299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2710225
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346251.2775692055087677No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC47320.1745980499774004No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG43720.16131501997066663No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42140.1554852456899335No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG41930.15471040227287403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117200.084.2967451
TAGGGCA12150.077.3644944
ACGGGTA2650.072.7153244
AGTAGGG43050.072.3856352
ATAGGGC14200.072.157283
GAATAGG16900.071.7869341
ATAGGGA21700.071.693583
AGGGCAT22800.071.5290765
ATAGCGG4750.071.2773061
TATAGGG21800.070.50232
GGATAGG15650.068.5066761
AGGGATG32650.068.231225
TAGGGTA8550.067.6124954
GTAGGGC10300.067.079863
GGTAAGG15200.066.822471
GTAGGGA19150.066.759363
TAGAGGG44950.065.2479552
ATAGAGG18000.065.048791
AGAGGGC35000.064.996413
ATGAGGG40750.064.937082
Produced by FastQC (version 0.11.5)