FastQCFastQC Report
Mon 27 Feb 2023
SRR3128298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366209
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311131.3148880762434765No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC43830.18523300350898841No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG38680.16346823125091656No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37470.15835456631261227No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG35720.15095876991423834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102300.084.7595141
ATAGCGG4350.078.9965441
TAGGGCA11450.074.378164
ATAGGGA18300.072.248293
TAGGGTA8300.070.8605044
GTAGGGA17300.070.713053
GTAGGGC9850.070.696493
GAATAGG11700.070.408771
TAGGGAT13600.070.23114
TAGAGGG39950.070.194292
AGGGATG29250.069.898425
AGTAGGG38050.069.3714142
ATAGGGC11450.068.2142263
GGTAAGG13600.068.187041
CGGGTAT2350.068.000965
CGAAGGG20950.067.825852
ACGGGTA2500.067.753984
TATAGGG18000.067.178912
GAGGGAT29400.066.416064
AGTAAGG14550.066.323191
Produced by FastQC (version 0.11.5)