FastQCFastQC Report
Mon 27 Feb 2023
SRR3128297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108276
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254071.205107870127061No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC44550.21131009412429874TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG43050.2041952761403156TruSeq Adapter, Index 16 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG40890.1939499382433799TruSeq Adapter, Index 16 (100% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTACCCTC36810.1745976333269458No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA31770.1506918449007625No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26100.1237978329213063No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23970.11369479138405028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85600.083.6317141
CCGTACA19700.078.962513
CGTACAC20000.077.543094
TAGGGCA10500.076.983624
TCCGTAC20800.076.594142
ACGGGTA1800.075.715484
ATCCGTA21000.075.705521
GAATAGG12400.072.450571
ATAGGGA19200.071.2280353
GGTAAGG12300.071.127571
TAGGGTA7550.070.960574
AGTAGGG34250.070.528022
ATAGAGG14200.069.2290041
CGGGTAT1850.068.5884865
AAGGGTA14050.068.2359164
AGGGATG25500.067.821415
TAAGGGA19700.067.750313
GTAGGGC9600.067.556483
CGTAGGG4600.067.428772
TAGCGGG9450.067.136882
Produced by FastQC (version 0.11.5)