FastQCFastQC Report
Mon 27 Feb 2023
SRR3128296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342678
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309021.3190886669017254No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC48550.2072414561454882TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG45470.19409410939104735TruSeq Adapter, Index 16 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG44110.188288787447528TruSeq Adapter, Index 16 (100% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30610.1306624299199463No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27590.1177712003100725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98450.084.40011
TAGGGCA11050.076.142564
GAATAGG12800.073.108061
ATAGAGG16050.072.6605761
ACGGGTA2350.072.006444
AGGGAAT20400.071.426445
CGAAGGG23100.068.981672
AGTAAGG14850.068.398961
AGTAGGG37950.068.123492
ATAGGGA20300.068.076513
ATAGGGC11300.067.803983
AGGGATG32200.067.585355
TAGGGTA8500.067.464864
GTAGGGC10250.067.4123463
ACGGGAT6500.067.2521744
TAAGGGA22800.067.20923
AAGGGTA17500.067.1488654
GGTAAGG13500.066.8789751
AGGGCAT21500.066.460345
ATGCGGG12100.066.428852
Produced by FastQC (version 0.11.5)