FastQCFastQC Report
Mon 27 Feb 2023
SRR3128295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062397
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268181.3003316044389126No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC39420.19113681798412235TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG39290.19050648347529597TruSeq Adapter, Index 16 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG37640.1825060839401919TruSeq Adapter, Index 16 (100% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26900.130430756057151No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23670.11476936787631091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87650.085.398621
ATAGGGA19850.074.1992653
ACGGGTA2350.074.088314
TAGGGCA10200.072.89084
TAGGGTA7400.071.855924
AGTAGGG33900.070.931152
TAAGGGA20650.070.413223
AGTAAGG12850.070.340221
GTAGGGA14550.069.856463
ATAGAGG14850.069.7431951
GAGGGAT24850.069.305954
GAATAGG11800.069.0191651
CGGGTAT1500.068.933615
AGGGATG27450.068.145985
CGAAGGG19200.068.133292
AGATAGG10550.067.826091
AGGGCAT19150.066.757445
AGAGGGC30250.066.7340853
TAGAGGG37500.066.631422
AAGGGAT36650.066.250864
Produced by FastQC (version 0.11.5)