FastQCFastQC Report
Mon 27 Feb 2023
SRR3128294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622657
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231731.4280898550956858No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC49840.30715055615573716No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGTCGCAT47090.29020304352675885No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG40700.25082318690887845No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG32530.2004736675711503No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27730.17089255461875183No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA25540.15739617183422006No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22150.13650451081158865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73500.086.413761
AGGGATG21850.076.569015
TAGGGCA8550.075.3024144
TAGCGGG8250.073.483562
CGTAGGG3400.073.257382
ATAAGGG22750.072.713612
ATAGGGA10550.072.608863
AGTAGGG25350.072.4858552
CGGGTAT1300.072.300465
GTAGGGA12300.071.8302153
GAGGGAT21400.071.810584
TCCGTAC21050.070.9952852
CCGTACA20550.070.664473
GTAGGGC7050.070.65963
TAGGGCG2000.070.492954
AGAGGGC22300.069.7553563
CGTACAC21000.069.150224
GGTAAGG9000.069.002981
ATAGCGG3550.068.9146351
AGGGAAT17400.068.602345