FastQCFastQC Report
Mon 27 Feb 2023
SRR3128292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536292
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243351.584008769166278No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC46940.3055408737401484No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG37030.24103490742645278No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG32150.20927011271294782No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25190.16396622517073578No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21410.13936152762625856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76450.086.233861
GAATAGG8700.076.318021
GTACGGG3600.075.839772
GTAGGGA12050.075.7855153
AGGGATG22600.073.412985
CGTAGGG3100.072.887162
AGGGAAT16700.072.3307955
ATAGGGA14050.071.363273
AGAGGGC20650.071.122343
GAGGGAT19550.070.549244
GGTAAGG8250.070.206681
AGTAGGG21950.069.269092
TAGCGGG7750.069.2428052
AAGAGGG51150.069.113962
TAAGGGA13150.069.0880663
TAGGGTA5550.068.701084
ATAGAGG12350.068.6329651
CGAAGGG15700.068.06092
AGGGACT25150.068.025225
AGATAGG10400.067.9180451
Produced by FastQC (version 0.11.5)