FastQCFastQC Report
Mon 27 Feb 2023
SRR3128291.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128291.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306634
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308671.3381836910407112No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC34760.15069577574942536No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG34610.15004547752265857No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCCCATGG33850.1467506331737068No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31150.13504526509190448No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29860.12945270034171003No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG29060.12598444313228713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99400.084.989431
AGATAGG13350.075.0905461
TAGGGCA11700.074.710694
CGTACAC15550.074.044264
ATAGGGC12000.072.059673
ATAGGGA18400.072.025613
TCCGTAC16700.071.75952
CCGTACA16400.071.352833
GAATAGG11900.070.7934341
CGTAGGG6050.069.910572
GAGGGAT25500.069.848124
TAAGAGG16100.069.572561
AGTTAGG6000.069.0268861
AGGGATG34550.068.690875
ATAAGGG28600.068.3572
TAGCGGG14800.067.952862
ATAGAGG18600.067.559311
AGTAGGG37650.067.403822
ATCCGTA17850.067.2339861
GTAGGGT8000.066.968493
Produced by FastQC (version 0.11.5)