FastQCFastQC Report
Mon 27 Feb 2023
SRR3128290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2565848
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365321.4237788052916618No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG38180.14880070838179035No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC37260.14521514914367492No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35730.13925220823680903No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG31600.12315616513526911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114550.083.667581
ATAGGGA19900.073.2183153
TAGGGCA11750.073.199384
ATAGAGG20100.071.827451
GGTAAGG13700.071.0555341
ATAGGGC14050.070.251253
GAGGGAT31350.069.412494
AGGGATG38000.069.1388855
GGATAGG15850.068.834611
AGATAGG15150.068.600581
GTACGGG6400.068.298952
TAGAGGG52350.068.145342
GAATAGG13400.067.7330551
AGTAGGG45400.067.29282
CGTAGGG7500.067.0553742
AGAGGGA59600.066.874533
TAAGAGG19850.066.809271
ATAGCGG6200.066.748141
TAAGGGA20250.066.150173
GTAGGGA19650.065.060513
Produced by FastQC (version 0.11.5)