FastQCFastQC Report
Mon 27 Feb 2023
SRR3128289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242785
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328771.4659006547662838No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG32850.14646967943873354No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC31530.1405841398083187No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30490.13594704797829485No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG26670.11891465298724577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102100.085.496921
GAATAGG12350.076.2481841
TAGGGCA10950.074.359014
CGTAGGG6950.073.137582
ATAGGGA19050.072.389283
ATAGGGC12050.071.867473
GAGGGAT27350.070.210884
AGTAAGG15550.069.640831
TAAGGGA18000.068.767763
GGTAAGG12450.068.0721741
AGGGATG34350.067.457575
TATAGGG17100.067.432862
TAAGAGG16500.067.343321
AAGGGAT34000.066.8605354
AGAGGGA48950.066.1511463
TAGGGTC6000.065.891544
AAGAGGG77950.065.752672
AGTAGGG36850.065.648872
GATAGGG32650.065.588822
GGATAGG13650.065.537131
Produced by FastQC (version 0.11.5)