FastQCFastQC Report
Mon 27 Feb 2023
SRR3128288.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128288.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840262
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397491.39948356876936No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC54210.19086267393641854TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG52720.18561667902468154TruSeq Adapter, Index 22 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCTAGTTG44900.15808400774294765No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG43420.1528732208507525TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37660.1325934015946416No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA35830.12615033401848139No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35240.1240730608655117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127650.085.117851
GAATAGG15100.075.103061
ATAGGGA25700.073.876673
TAGGGTA8850.073.2815864
AGTAGGG43350.073.176852
CCGTACA20800.072.5271763
CGTACAC20900.072.405024
CGTAGGG8050.072.3910452
AGGGTAC13250.072.0011145
TCCGTAC21450.071.643952
GGTAAGG15100.070.740231
AGTAAGG16450.070.655921
ATCCGTA22150.069.893971
AGGGCAT23500.069.793765
TAGCGGG13600.069.457032
AGGGATG38050.069.2894745
ATAGGGC15250.069.02993
GAGGGAT33650.068.852694
GTAGGGA20200.068.6324843
TAGGGCA13950.068.388164
Produced by FastQC (version 0.11.5)