FastQCFastQC Report
Mon 27 Feb 2023
SRR3128287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162997
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT486331.5375607374904243No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC62760.19841941045154327TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG60550.19143236620205456TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG47850.15128057345612406TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42620.13474562258516212No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41880.1324060693070528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150750.084.4892041
TAGCGGG18450.073.88052
GAATAGG15350.073.5381551
AGTAGGG52700.073.2252352
ATAGGGA26950.073.077473
TAGGGCA14800.072.7269064
ATAGGGC15350.072.265643
AGGGTAC14050.071.924475
AGTAAGG18350.071.768161
GTAGGGA24800.070.504943
AGGGATG41800.069.71565
TAGAGGG60600.069.574182
TAGGGTA9950.068.023784
CCGTACA15450.067.842933
GGTAAGG18550.067.19111
GAGGGAT38500.067.024414
AGGGCAT27050.066.723455
TCCGTAC16150.066.648632
ATAGCGG6850.066.602581
ATAAGGG43350.066.574442
Produced by FastQC (version 0.11.5)