FastQCFastQC Report
Mon 27 Feb 2023
SRR3128286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780548
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423601.5234407030556567No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC48680.17507340279685873TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG48020.17269976997340092TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG38710.13921716150917013TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38610.1388575201722826No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36000.12947088127951756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT135500.085.2392961
TAGGGTA9750.075.31144
TAGCGGG15050.073.810182
GAATAGG15150.072.725181
ATAGGGA23100.072.132683
CGTAGGG8400.071.725152
ATAGAGG19200.071.1178051
TAGAGGG50350.070.768042
AGTAGGG46600.070.503432
AGTAAGG14300.070.462711
TAGGGCA14950.070.2108844
ATAGGGC14350.069.538413
GAGGGAT33750.069.174924
AGGGCAT25600.069.031565
GTAGGGC12000.068.643213
TAAGGGA21950.068.1919863
GTAGGGT11750.068.100743
TAGACGG3400.067.8576351
AGGGATG38150.067.3895655
AAGAGGG98250.067.358672
Produced by FastQC (version 0.11.5)