FastQCFastQC Report
Mon 27 Feb 2023
SRR3128285.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128285.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212795
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281171.2706554380319912No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGCCCAGA40290.18207741792619742No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC29820.13476169279124367Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG28660.12951945390332137No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA28430.12848004446864714No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG25320.11442542124326927No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24030.10859569006618326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93000.085.654891
TCCGTAC19550.076.686922
CCGTACA19150.075.834543
CGTAGGG7900.074.958492
ATAGGGA21150.073.996513
ATCCGTA20800.072.840131
AGGGATG33450.072.4988255
GGTAAGG13550.072.227441
AGTAGGG36700.072.225482
GAGGGAT29550.072.206414
GAATAGG13600.071.615931
TAGGGAT17000.071.049594
CGTACAC20400.070.26634
TAGCGGG10500.069.825282
ATAGGGC12450.069.458573
GTAGGGA17550.068.5551763
TAGGGCA11200.068.398574
ATAAGGG29000.068.3898852
TAGAGGG39200.068.098832
TAAGGGA21000.068.034893
Produced by FastQC (version 0.11.5)