FastQCFastQC Report
Mon 27 Feb 2023
SRR3128284.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128284.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463365
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335671.3626482474176584No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG30180.122515339789272No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC29160.11837466230136418Illumina Single End Adapter 2 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28350.1150864772374374No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG26740.10855070198691626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109700.085.187341
TAGACGG3050.075.557921
CGTAGGG8000.073.441122
ATAGGGA25150.072.512513
GAATAGG15800.072.332421
AGGGATG36900.071.965395
TAGGGCA14300.071.652434
AGTAGGG46050.071.549672
ATAACGG1850.071.1819841
TAGGGTA9850.069.666994
GGTAAGG15400.069.324851
GTAGGGA20500.069.242433
TAGCGGG12000.068.936732
TAGGGAT20150.068.810924
CCGTACA12150.068.472513
TAAGGGA22650.067.442623
GTACGGG6100.067.036082
ATAGGGC14450.066.681483
AAGAGGG91550.066.5374152
AAGGGAT45500.066.421714
Produced by FastQC (version 0.11.5)