FastQCFastQC Report
Mon 27 Feb 2023
SRR3128283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135964
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298381.3969336561852166No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG26650.12476802043480133No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC26380.12350395418649378Illumina Single End Adapter 2 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23030.10782016925378893No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG22880.10711791022695138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95100.086.710861
TAGGGCA12550.077.976984
ATAGGGA20700.076.141483
GAGGGAT30700.072.641844
CGTAGGG7050.071.407122
ATAGGGC12850.071.0305943
AGGGATG34350.070.878165
AGTAGGG38200.070.572932
TAGGGAT15150.070.184734
TAGAGGG40100.068.754332
GGTAAGG12200.068.672061
TAGACGG2950.068.6066741
GTAGGGA17950.068.148413
AGGGCAT20200.067.9424065
AAGAGGG76650.067.703362
GAATAGG13750.067.092391
TAGGGAC12700.066.6830754
AAGGGAT37450.065.453484
AGGGATT23200.065.437095
AGAGGGA55750.064.728773
Produced by FastQC (version 0.11.5)