FastQCFastQC Report
Mon 27 Feb 2023
SRR3128282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844884
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223571.211837709037533No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAGTCTTC83470.4524403702346597No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG68760.37270635985785555Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC67950.36831583991188604TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG66940.3628412409669117Illumina PCR Primer Index 7 (95% over 22bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA41250.2235912935447432No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28940.1568662311559968No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATG24910.13502203932604975No Hit
AGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT22180.12022436098963404No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21960.11903187409072874No Hit
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT21820.11827301879142538TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT20790.11269001194655058TruSeq Adapter, Index 23 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG19440.10537247870326806TruSeq Adapter, Index 23 (95% over 24bp)
AAAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT19370.10499305105361638TruSeq Adapter, Index 23 (95% over 21bp)
GGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT19080.10342113650505938TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74900.085.251051
TAGGGCA8050.075.891934
TAGCGGG7700.075.679652
TCCGTAC37100.074.22872
GTAGGGC7500.073.93823
CCGTACA35100.073.772183
ATCCGTA38050.073.2097241
ATAGGGA17100.072.55313
AGTAGGG28700.072.5387342
CGTACAC35800.071.93594
AGGGAAT19900.071.790785
AAGGGAT29200.071.618514
ATAGCGG3100.071.340781
GTAGGGA15500.071.249913
AGTAAGG11750.070.882191
TAGGGAT13500.069.9697654
AGGGATT17550.069.8894355
GCATAGG3050.069.424761
TATAGGG14800.069.22182
TAAGGGA17100.068.980413
Produced by FastQC (version 0.11.5)