FastQCFastQC Report
Mon 27 Feb 2023
SRR3128281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035233
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281271.382003927805809No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC78500.38570522392276463TruSeq Adapter, Index 23 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG78030.38339590602157103Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG70150.34467798035900554Illumina PCR Primer Index 7 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAGTCTTC50920.25019248410378564No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33550.16484599060648092No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA30010.1474524047123843No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25710.12632460263763412No Hit
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT24940.12254125203355094TruSeq Adapter, Index 23 (95% over 23bp)
AGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT24610.12091981606037244No Hit
AAAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT22340.10976630194184155TruSeq Adapter, Index 23 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT21270.10450891863486883TruSeq Adapter, Index 23 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG20530.10087297130107463TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94300.084.9724351
ATAGCGG3550.079.4772951
TAGCGGG8900.075.5183942
TAGGGCA8050.074.150584
CGTAGGG4950.073.11262
AGGGATG25700.072.238835
ACGGGTA2350.072.0014654
AAGGGAT29500.071.8557054
AGTAGGG30900.071.185912
ATAGGGA20750.071.1243443
GTAGGGT7750.070.956283
AGGGATT20050.069.622375
TAGGGAT14850.069.3145454
AGGGAAT22200.068.807715
TAGAGGG31300.068.173912
AGAGGGC25600.067.931073
AAGAGGG60250.067.7125852
GTAGGGA15850.067.610213
AAGGGCG9250.067.579764
ATAGAGG11900.067.572391
Produced by FastQC (version 0.11.5)