FastQCFastQC Report
Mon 27 Feb 2023
SRR3128280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789826
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239501.3381189009434435No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG63520.3548948333525158Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC60770.33953021131663075TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG60530.33818929884804444Illumina PCR Primer Index 7 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29910.1671112163975716No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAGTCTTC26950.1505732959516735No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20840.11643589935557981No Hit
AGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT20740.11587718582700217No Hit
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT20500.11453627335841585TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT19960.11151922030409661TruSeq Adapter, Index 23 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT18230.10185347625970345TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80600.087.324921
ACGGGTA2500.075.304054
ATAGGGA17250.074.485533
AGTAGGG28750.073.012192
AGGGAAT19600.072.6590965
CGTAGGG4050.072.050172
GAATAGG9700.070.861961
TAGCGGG8450.070.736792
ATAGCGG3600.070.619281
GTAGGGA16100.069.866733
TAGGGAC11400.069.771844
AAGGGTA12350.069.740094
GGTAAGG10000.069.677691
AAGGGAT28200.069.596164
CGAAGGG16250.069.22182
AGGGATG25950.069.187785
AGGGACT30050.068.819525
AGAGGGA42900.068.787353
TAGGGCA7400.068.689514
GTAGGGT6050.068.458233
Produced by FastQC (version 0.11.5)