FastQCFastQC Report
Mon 27 Feb 2023
SRR3128279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398496
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACAGCGAA101542.5480807837468884No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG44281.1111780293905082No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44011.1044025536015418No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA37010.9287420701838914No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC36150.9071609250782944No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATG35420.8888420460933109No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG22610.5673833614390107No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCACAGCGAATC22490.5643720388661366No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT16150.40527382959929337No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCACAGCGAATCGT14490.36361720067453623No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGC14420.36186059584035973No Hit
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG13870.34805870071468725No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG13730.3445454910463342No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG13660.34278888621215775No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG11810.2963643298803501No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCACAGCGAATCG10560.26499638641291257No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCG9980.2504416606440215No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG7840.19673974142776843No Hit
ATCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC7630.1914699269252389No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG7100.17816991889504535No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC6890.17290010439251585No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC6550.16436802376937285No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT5930.1488095238095238No Hit
GACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC5700.14303782221151531No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT5390.13525857223159077No Hit
ATCCGTACACCCACACACCGAATCCTGTCTCTTATACACATCTGACGCAC5350.13425479804063278No Hit
AGGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT5310.13325102384967477No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT5230.13124347546775877No Hit
ACACCGAGATCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATG5220.13099253192001928No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT5160.12948687063358227No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG5040.12647554806070826No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTA4980.12496988677427126No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT4780.11995101581948125No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4450.11166987874407773No Hit
ACACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4410.11066610455311975No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4390.11016421745764073No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC4310.10815666907572472No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCACAG4200.10539629005059022No Hit
GGAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4110.10313779812093471No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA156.90239E-493.987958
CGCGATC156.90239E-493.987955
AGGGTCG156.90239E-493.987955
CGTTTTT34400.088.0655061
ATAGCGG550.085.529461
TCACGGG401.035005E-982.239462
ATCCGTA43150.078.819911
TCCGTAC42950.078.560362
GTAGCGG600.078.402011
CATACGG301.4468296E-678.402011
CGAGGGA1500.078.3232963
CCGTACA41250.078.038483
CGTACAC41350.077.736114
GTACACC41650.077.0633545
TAGGGCA1650.076.899244
TACACCC39800.076.394736
ACGGGAA1050.076.085494
GTAAGGG4800.075.386172
TAGCGGG1500.075.190362
TCCGTAA255.558451E-575.190362
Produced by FastQC (version 0.11.5)