FastQCFastQC Report
Mon 27 Feb 2023
SRR3128278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425471
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACAGCGAA59681.402680793755626No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54781.2875143076731435No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG50971.1979664889028863No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC38930.9149859802430718No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA26280.6176684192342133No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG24300.5711317575110877No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATG20230.47547306396910716No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT17880.4202401573785287No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG14950.3513752993741054No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG14090.3311624058984044No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCACAGCGAATC13060.30695394045657637No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG12820.30131313297498535No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCACAGCGAATCG11950.28086520585421804No Hit
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG10880.25571660583212485No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG8160.19178745437409367No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCACAGCGAATCGT7920.18614664689250265No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGC7610.1788606038954476No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC7500.17627523379971843No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG7320.1720446281885252No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT6590.15488717209868594No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT6310.14830623003682977No Hit
GACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC6290.1478361627466972No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC6210.14595589358616687No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT5940.139609985169377No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT5880.13819978329897925No Hit
ATCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC5830.1370246150736478No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG5820.1367895814285815No Hit
AGGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT5710.1342042113328523No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT5280.12409776459500177No Hit
ACACCGAGATCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATG5180.12174742814433887No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTA5100.11986715898380854No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC5070.11916205804860965No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCG5050.11869199075847708No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4870.11446138514728382No Hit
ACACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4650.10929064495582541No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCACAG4640.10905561131075914No Hit
GGAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4470.10506003934463218No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4450.10458997205449959No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT4310.10129950102357153No Hit
GGGGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4270.10035936644330636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG600.094.0921
GGCCGAT254.9147275E-794.003527
CGTTTTT41850.090.49471
TAGCGGG1850.088.922262
GTAGCGG500.084.68281
GTACGGG1000.084.6031652
AGGGTAC1300.083.156975
CGTAGGG353.8577127E-880.5744552
TTACGGG700.080.5744552
CGAAGGG2050.080.246912
AAGGGTC1700.080.179484
CGGGAAT650.079.541445
TAGGGTG600.078.3362664
CGGGTAC551.8189894E-1276.911975
TACGGGA1100.076.911973
GTATAGG800.076.449751
AGTACGG800.076.449751
GGTAAGG2500.075.2736051
TTAACGG255.5284818E-575.27361
ATAGGGT1250.075.202823
Produced by FastQC (version 0.11.5)