FastQCFastQC Report
Mon 27 Feb 2023
SRR3128277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362518
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45831.2642130873501454No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG40411.1147032699065977No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC31520.8694740674945797No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACAGCGAA31040.8562333456545606No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG20150.55583446890913No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT15520.4281166728272803No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG15160.4181861314472661No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG14840.4093589835539201No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14480.39942844217390583No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG12040.33212143948714273No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCACAGCGAATCG10260.28302042933040567No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATG9840.27143479772038903No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG8090.22316133267865318No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG7920.21847191036031316No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCACAGCGAATC6760.18647349924693393No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC6500.17930144158359035No Hit
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG6290.17350862577858203No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC5640.15557848162022297No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG5590.15419923976188768No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT5370.1481305755852123No Hit
GACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC5110.1409585179218687No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT5060.13957927606353338No Hit
AGGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4990.13764833746186397No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT4760.13130382491352155No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTA4630.12771779608184972No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT4540.12523516073684618No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC4520.12468346399351206No Hit
ATCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC4460.12302837376350967No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT4040.11144274215349308No Hit
ACACCGAGATCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATG3960.10923595518015658No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGC3930.10840841006515539No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT3910.10785671332182126No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCACAGCGAATCGT3880.10702916820682007No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCACAG3800.10482238123348357No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT3730.10289144263181416No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGG3680.10151220077347883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA353.5106495E-1094.260621
TTTACGG301.2982127E-894.260621
TGTACGG156.8228575E-494.260621
CGGGTAC201.831112E-594.052455
TAGCGGG1150.090.0875552
CGTTTTT37650.088.251971
ATAGCGG550.085.691471
AGGGTAC950.084.152195
TTACGGG900.083.7177352
GGTAAGG2550.081.322881
TTAACGG353.78368E-880.794821
ATTGCGG650.079.758981
GTAGAGG1900.079.377361
AGGGATT3400.078.838085
GAATAGG1800.078.5505141
GTATAGG1500.078.5505141
CGAAGGG2300.077.802892
AGGGCGA1650.076.9525
AAGGGCA4800.075.5421754
ATAGGGC1250.075.3459553
Produced by FastQC (version 0.11.5)