FastQCFastQC Report
Mon 27 Feb 2023
SRR3128276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909583
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250331.3109144771397736No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG60970.31928436731998555No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTACATCA59400.31106267703472434No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC58460.30614013635437687No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG56460.2956666455451269No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36920.1933406403387546No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA36860.19302643561447708No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21580.11300896583180725No Hit
AGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT19530.10227363775232602No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATG19390.10154049339567853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81250.086.613531
CCGTACA27350.079.7333453
TCCGTAC28500.078.3299942
CGTACAC28300.076.890714
ATCCGTA29400.076.4818041
TAGGGCG3100.074.287164
CGTAGGG4850.072.677312
TAGGGCA7400.072.402324
CGGGTAT850.071.8792955
CGAAGGG16600.070.4969942
AGGGAAT16500.070.3545765
TAAGGGA15950.070.128673
ACGGGAT4900.070.0174264
AGGGCAT17000.069.944085
AGTAAGG11050.069.816661
ATAGCGG2700.069.690531
GGTAAGG10500.069.4416351
GAATAGG9100.069.2693251
GTACACC31300.068.920395
ATAGAGG11400.068.498451