FastQCFastQC Report
Mon 27 Feb 2023
SRR3128274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862462
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260791.4002433338237237No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG56210.3018048153465681No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC51070.27420693683951675No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG50470.2709853946013395No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37150.19946715691380548No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20820.11178751566474913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83700.087.083771
ATAACGG1750.080.718841
TAGGGCA9350.078.531014
ATAGCGG3050.074.102551
AGGGCAT15650.073.574375
AGGGAAT16750.072.1096045
CGTAGGG5300.071.9364552
ACGGGAT4350.071.413924
TATAGGG14950.070.840442
TAAGGGA15600.070.60243
GTAGGGC6800.069.9102253
ATGCGGG9050.069.6941152
AGTAGGG27850.069.6324842
GAATAGG9400.069.627161
TAGAGGG31050.068.974682
CGAAGGG17600.068.464782
ATAAGGG22300.068.388032
CGGGTAT1100.068.360015
ATAGGGA18050.068.32073
GGTAAGG10200.067.859231
Produced by FastQC (version 0.11.5)