Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128271.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1645917 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 23383 | 1.4206670202689442 | No Hit |
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG | 5223 | 0.31733070379612094 | No Hit |
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG | 5186 | 0.31508271680771266 | No Hit |
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC | 4685 | 0.2846437578565626 | TruSeq Adapter, Index 13 (95% over 21bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2523 | 0.15328841004740823 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACAATCCC | 2099 | 0.12752769428835112 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2052 | 0.12467214324902168 | No Hit |
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT | 1755 | 0.10662749093666327 | No Hit |
AGAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT | 1661 | 0.10091638885800437 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 7635 | 0.0 | 88.893005 | 1 |
TAGGGCA | 750 | 0.0 | 81.564476 | 4 |
TAGGGCG | 230 | 0.0 | 75.69946 | 4 |
CGTAGGG | 390 | 0.0 | 74.807655 | 2 |
GTAGGGT | 655 | 0.0 | 73.27871 | 3 |
AGTAGGG | 2525 | 0.0 | 73.05393 | 2 |
TAGGGTA | 575 | 0.0 | 72.83516 | 4 |
AGGGATG | 2080 | 0.0 | 71.639534 | 5 |
AGGGCAT | 1560 | 0.0 | 71.413536 | 5 |
ATAAGGG | 2065 | 0.0 | 71.325226 | 2 |
GTAGGGC | 715 | 0.0 | 71.07824 | 3 |
GGTAAGG | 890 | 0.0 | 70.86408 | 1 |
AGGGAAT | 1405 | 0.0 | 70.59321 | 5 |
GTAGGGA | 1115 | 0.0 | 69.21304 | 3 |
AGGGTAC | 780 | 0.0 | 68.70163 | 5 |
GCATAGG | 475 | 0.0 | 68.37021 | 1 |
AGTAAGG | 770 | 0.0 | 67.84903 | 1 |
ATAGGGA | 1235 | 0.0 | 67.44119 | 3 |
TAAGGGA | 1235 | 0.0 | 67.44119 | 3 |
CGAGGGA | 775 | 0.0 | 67.39694 | 3 |