FastQCFastQC Report
Mon 27 Feb 2023
SRR3128271.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128271.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645917
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233831.4206670202689442No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG52230.31733070379612094No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG51860.31508271680771266No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC46850.2846437578565626TruSeq Adapter, Index 13 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25230.15328841004740823No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACAATCCC20990.12752769428835112No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20520.12467214324902168No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT17550.10662749093666327No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT16610.10091638885800437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76350.088.8930051
TAGGGCA7500.081.5644764
TAGGGCG2300.075.699464
CGTAGGG3900.074.8076552
GTAGGGT6550.073.278713
AGTAGGG25250.073.053932
TAGGGTA5750.072.835164
AGGGATG20800.071.6395345
AGGGCAT15600.071.4135365
ATAAGGG20650.071.3252262
GTAGGGC7150.071.078243
GGTAAGG8900.070.864081
AGGGAAT14050.070.593215
GTAGGGA11150.069.213043
AGGGTAC7800.068.701635
GCATAGG4750.068.370211
AGTAAGG7700.067.849031
ATAGGGA12350.067.441193
TAAGGGA12350.067.441193
CGAGGGA7750.067.396943