FastQCFastQC Report
Mon 27 Feb 2023
SRR3128270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174364
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389171.789810721663898No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG31460.14468598633899382No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28890.1328664381860627No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC26780.123162451181127No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT24690.11355044509566935No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22780.10476626728551429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121050.086.243211
CCGTACA10400.075.46683
ATAGGGC12350.074.2062453
GAATAGG12550.073.475871
TCCGTAC11300.072.783412
ATAGGGA17600.071.029993
GTAGGGT7300.068.242643
TAGGGTA6500.067.965314
AGTAGGG35200.067.825632
CGTAGGG6450.067.763532
ATCCGTA12250.067.594131
AGGGTAC7650.067.57785
CGTACAC11550.067.545884
GCATAGG6200.067.5352861
ACGGGTA2300.067.430894
TATAGGG17850.067.402272
AAGAGGG75000.067.049462
AGATAGG12250.066.826021
TAAGGGA18150.066.806063
GGTAAGG13550.066.664461
Produced by FastQC (version 0.11.5)