FastQCFastQC Report
Mon 27 Feb 2023
SRR3128269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436901
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464471.905986332641334No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG34300.14075253775184138No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33180.13615653651912818No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC29250.12002949647933994No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT26920.11046817248628482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT142100.085.858881
ATAGGGC15050.072.4532243
TAGGGCA14700.071.9401554
GAATAGG14050.070.9690251
AGTAGGG41800.069.82672
ATAGGGA21000.068.487043
AGGGCAT23000.068.049135
AGGGATG34550.067.882635
AGAGGGC37100.066.510713
TATAGGG21100.066.157672
TAGAGGG45800.065.780742
GAGGGAT28700.065.670254
AAGAGGG87050.065.6014942
AGTAAGG15300.065.478311
ATAGCGG4750.065.352111
ATAAGGG33950.065.3444062
TAGGGTA8500.064.695364
GTAGGGA16300.064.5901953
AAGGGGC75300.064.540414
GCATAGG7450.064.395161
Produced by FastQC (version 0.11.5)