FastQCFastQC Report
Mon 27 Feb 2023
SRR3128268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096327
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408151.9469767836792637No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28970.1381940889947036No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG28320.13509342769520213No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC24580.11725269960268603No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT23550.11233934400501448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129750.086.0427861
GAATAGG12800.073.939481
ATAGGGC10850.072.879243
CGTAGGG6050.068.462322
TAGAGGG40850.068.441372
TAAGGGA16500.068.1770553
AGTAGGG34100.067.3580862
ATAGCGG4900.067.265481
GTAGGGT7300.067.055563
ATAGGGA17250.066.8499763
GGTAAGG12000.066.704931
AGGGATG30300.066.545235
CGAAGGG24050.066.345112
AGGGCAT18750.065.675345
AAGAGGG75200.065.469372
TAGGGTA7000.065.222594
TAGGGCA10200.065.064374
ATAAGGG29000.064.758862
AAGGGAC21500.064.581464
TGTAGGG13550.063.9150052
Produced by FastQC (version 0.11.5)