FastQCFastQC Report
Mon 27 Feb 2023
SRR3128267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666679
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376031.4101059782598506No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC76460.2867236739030082TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG69390.26021129652275354No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG65630.24611136173495196No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATAGGAC55560.20834903638570673No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA48980.1836741505070539No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37110.13916185637641426No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35800.13424937909662168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112600.086.605521
CCGTACA26800.076.983733
TCCGTAC28050.076.4013752
ATCCGTA28250.075.766571
TAGGGCA15450.073.6132664
CGTACAC28000.073.348734
CGTAGGG8950.071.939382
AGGGATG35050.071.601555
ATAGCGG4350.071.3738861
ATAGGGA22850.069.3127063
AAGGGAT37750.068.9702764
GAATAGG15300.068.871771
GTACGGG6350.068.830092
AGAGGGC40000.068.497973
ACTAGGG11350.067.907412
TAGGGTA7550.067.849834
ATAGAGG16050.067.705141
AGTAGGG41350.067.057242
ATAAGGG34350.067.0406952
AGATAGG15250.066.9382861
Produced by FastQC (version 0.11.5)