FastQCFastQC Report
Mon 27 Feb 2023
SRR3128266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957030
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT456401.5434405467648282No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC86060.291035261732211TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG74350.25143471659063316No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG69110.2337142335383814No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42100.14237258330148833No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40480.13689411335021964No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA34440.11646821303808214No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATAGGAC34110.11535222841837925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT128300.086.716151
TAGGGCA13750.071.780774
ATAGGGA24150.071.619223
AGGGATG39500.070.914225
TAGGGCG5300.070.055584
AGATAGG14900.069.1145861
AGTAGGG43600.069.0987852
CGTAGGG9200.068.967552
GAATAGG17800.068.421361
TAGGGTA7950.067.986434
ATAGGGC17600.067.562663
AAGGGAT42650.067.5511864
GAGGGAT33750.067.539764
ATAGAGG20150.067.4426651
GTAGGGT8800.067.2956163
ACGGGTA2450.067.141884
AGGGCAT24750.067.0322345
TAGACGG2750.066.68741
GTAGGGC14300.066.391763
ATAAGGG38450.065.885722