Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128266.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2957030 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 45640 | 1.5434405467648282 | No Hit |
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC | 8606 | 0.291035261732211 | TruSeq Adapter, Index 21 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG | 7435 | 0.25143471659063316 | No Hit |
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG | 6911 | 0.2337142335383814 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 4210 | 0.14237258330148833 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 4048 | 0.13689411335021964 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 3444 | 0.11646821303808214 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATAGGAC | 3411 | 0.11535222841837925 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 12830 | 0.0 | 86.71615 | 1 |
TAGGGCA | 1375 | 0.0 | 71.78077 | 4 |
ATAGGGA | 2415 | 0.0 | 71.61922 | 3 |
AGGGATG | 3950 | 0.0 | 70.91422 | 5 |
TAGGGCG | 530 | 0.0 | 70.05558 | 4 |
AGATAGG | 1490 | 0.0 | 69.114586 | 1 |
AGTAGGG | 4360 | 0.0 | 69.098785 | 2 |
CGTAGGG | 920 | 0.0 | 68.96755 | 2 |
GAATAGG | 1780 | 0.0 | 68.42136 | 1 |
TAGGGTA | 795 | 0.0 | 67.98643 | 4 |
ATAGGGC | 1760 | 0.0 | 67.56266 | 3 |
AAGGGAT | 4265 | 0.0 | 67.551186 | 4 |
GAGGGAT | 3375 | 0.0 | 67.53976 | 4 |
ATAGAGG | 2015 | 0.0 | 67.442665 | 1 |
GTAGGGT | 880 | 0.0 | 67.295616 | 3 |
ACGGGTA | 245 | 0.0 | 67.14188 | 4 |
AGGGCAT | 2475 | 0.0 | 67.032234 | 5 |
TAGACGG | 275 | 0.0 | 66.6874 | 1 |
GTAGGGC | 1430 | 0.0 | 66.39176 | 3 |
ATAAGGG | 3845 | 0.0 | 65.88572 | 2 |