FastQCFastQC Report
Mon 27 Feb 2023
SRR3128265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599037
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394791.518985685852106No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC67090.25813407042685427TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG63830.24559096311441508No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG58250.224121472683921No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37360.14374554883212512No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35840.1378972288582271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117700.086.858151
TAGGGCA13750.074.6057364
CGTAGGG9150.072.51312
ATAGGGC16050.070.657863
AGGGATG34150.070.463245
ATAGGGA22950.069.713143
TATAGGG20650.069.5021442
AGTAGGG39650.069.190082
GTAGGGA19950.068.874493
GGTAAGG15900.068.678071
ATAGAGG16700.068.206551
TAGGGTC7050.068.081564
GAATAGG16250.068.06781
GTAGGGT8800.067.91093
ATAGCGG4450.067.693531
TAGAGGG42000.067.335432
GTACGGG5200.066.964842
AGGGCAT21300.066.8569265
ACGGGAT8600.066.754394
GAGGGAT27700.066.5924154
Produced by FastQC (version 0.11.5)