FastQCFastQC Report
Mon 27 Feb 2023
SRR3128263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311426
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265411.148252204483293No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC53420.23111274165817985No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG52370.22657009136351328No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG48920.21164424039532306No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37220.16102613711189542No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAATGACAG30060.1300495884358833No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29600.12805947497345793No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27450.11875785770342638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92450.085.865171
TAGCGGG12450.076.258662
ATAGCGG5800.073.784461
TAAGGGA20500.070.845413
ATAACGG3000.070.541181
AAGGGTA14250.070.2541354
AGGGCAT23350.070.04715
GTAGGGT10850.069.743433
CGTAGGG5400.069.631122
GGTAAGG14450.069.320751
GTACGGG4500.068.934812
TAGGGCA12500.068.809474
AGAGGGC31900.068.807113
TAGGGCG3350.068.747744
AGTAGGG39600.068.721172
AAGGGAC23050.068.309494
CGAAGGG21100.068.16262
AAGAGGG78750.067.323352
AGGGATG31750.066.762195
ATAGGGC15300.066.661563
Produced by FastQC (version 0.11.5)