FastQCFastQC Report
Mon 27 Feb 2023
SRR3128262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031075
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230501.134867003926492No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC42710.21028273205076128No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG40790.2008296099356252No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG39830.19610304887805718No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32050.15779821030734956No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25840.12722326846620632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85550.086.6447451
ATAGCGG4700.073.144641
GTACGGG4200.072.860652
TAGGGCA12250.072.636464
TAGGGCG3000.072.188094
CGTAGGG5550.072.1032642
AGGGCAT21300.071.04875
AGTAGGG32950.070.583062
CGAAGGG19850.069.966552
TAGAGGG37200.069.859442
AGGGATG27750.069.438575
GAATAGG12050.068.39251
ATAAGGG28800.068.330212
AAGGGTA14900.067.933064
TAAACGG2500.067.81411
AGGGTAT12200.067.8004765
AGCAGGG36950.067.529012
AAGAGGG68300.067.482472
AGAGGGC28550.067.444443
TAGCGGG10750.067.443672
Produced by FastQC (version 0.11.5)