FastQCFastQC Report
Mon 27 Feb 2023
SRR3128261.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128261.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477867
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324841.310966246372384No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC52940.21365149945497477TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG43350.17494885722276457No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGTGCTT40180.16215559592181503No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG39500.15941130012224225No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA37090.14968519295022695No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28990.11699578710237474No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27100.10936825907120921No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106200.085.597561
TAGGGCA11650.075.438774
CCGTACA20600.075.059843
TCCGTAC21250.074.5332262
TAGGGTA8600.074.322344
CGTACAC21100.072.835694
ATCCGTA22300.071.527581
ATAGGGA21500.070.824823
GTAGGGC10400.070.4969253
TAAGGGA21950.070.229293
AGGGATG35150.070.196095
TAGCGGG13150.069.692792
ATAGGGC14200.069.504013
AGAGGGC33800.069.24553
ATAGCGG5650.069.120741
ACGGGAT7500.068.303694
AGTAGGG40450.068.202222
GTAGGGA16100.068.015673
CGTAGGG7200.067.8859252
AAGGGTA18050.067.177134
Produced by FastQC (version 0.11.5)