FastQCFastQC Report
Mon 27 Feb 2023
SRR3128260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760338
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391291.4175437935499202No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC57620.20874255254247848TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG48890.17711599086778504No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG41590.15066995418676987No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32530.1178478867443045No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29160.10563923693402764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125050.085.4944841
TAGGGCA14750.077.755114
CGTAGGG8000.075.793322
GTAGGGC12400.072.400713
ATAGGGC16450.072.005523
ATAGCGG6400.070.55271
AGGGATG37550.069.846975
TAGCGGG16150.069.559522
ATAGGGA23350.069.2472843
AGAGGGC40100.068.805783
TAGAGGG48750.067.781612
TAGACGG3900.067.537631
CGAAGGG30350.067.3692552
AGTAGGG43900.067.239782
AGGGTAT15750.067.146795
ATAGAGG20750.066.869221
ACGGGTA3700.066.059124
CCGTACA14750.065.964383
ACGGGAT7800.065.684534
AGGGCAT23350.065.622695
Produced by FastQC (version 0.11.5)