FastQCFastQC Report
Mon 27 Feb 2023
SRR3128259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428185
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342651.4111363013938394No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC46270.19055384989199753TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG39280.1617669164417044No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG34970.1440170332985337No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27490.11321213169507266No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26800.11037050307122398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108500.085.2955861
TAGGGCA14750.076.252354
ACGGGAT7150.071.7410664
ATAGGGA22900.071.7195053
CGTAGGG5000.071.530462
TAGGGTA8150.071.022324
GAATAGG15950.070.830991
GTAGGGC11400.070.589263
ATAGGGC15650.070.363743
ATAGCGG5200.068.799141
AGTACGG2900.068.174831
CGAAGGG24950.068.090112
AGGGATG35600.067.9921345
ACGGGTA3050.067.889134
TATAGGG21250.067.544232
TAGCGGG12900.067.1236342
AGAGGGC36150.066.911723
TAAGGGA22750.066.60733
TAGAGGG45650.066.3884352
TAGACGG3900.066.385141
Produced by FastQC (version 0.11.5)