FastQCFastQC Report
Mon 27 Feb 2023
SRR3128258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716812
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210561.2264592745157887No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC52310.3046926512629222No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG51760.301489038986214No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACACAACT51080.29752820926228385No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG50120.2919364496520295No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA37120.21621470492983508No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29660.1727620729584835No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25580.1489970946149025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69950.086.36571
TAGCGGG7700.079.9513242
CGTAGGG6150.077.177622
CCGTACA24350.075.0750663
TCCGTAC25650.074.934372
AGGGCAT14300.074.599335
ATAGCGG4050.073.189631
ATCCGTA26250.072.592161
GTAGGGC8100.072.522063
CGTACAC25100.072.457334
ATAGGGA15400.071.712073
AGTAGGG25950.071.351642
GGTAAGG10800.071.011361
CTACGAC6750.070.317385
TAAGGGA15150.070.10373
AGGGATG26350.069.911815
GTAGGGA14800.069.538853
ATAAGGG21200.069.383082
GACCGAT950.069.2547458
TAGGGTA5650.069.035864