FastQCFastQC Report
Mon 27 Feb 2023
SRR3128257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895891
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257351.3574092603424988No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC59870.315788196684303No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG59420.3134146425084564No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG52890.27897173413450455No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33120.1746935873423103No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACACAACT29710.1567073212542282No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28880.15232943244100003No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24770.1306509709682677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84800.086.836351
TAGCGGG8650.082.587722
ATAGCGG3200.076.411551
GGTAAGG10500.074.3403241
TAGGGCG2800.073.855464
AGGGCAT16550.072.1312945
CTAGCGG3350.071.586491
ACGGGTA2700.071.368754
AGGGATG30000.070.968385
TAAGGGA16150.070.716663
CGTAGGG7300.070.176482
AAGGGAT34350.069.91694
AGGGATC18900.069.876725
TAGGGTC5550.069.4398654
ATAGGGA18750.069.433093
TAGGGCA11050.069.328744
AGGGAAT19350.069.2232365
CCGTACA16500.068.931763
GTACGGG3750.068.931762
TATAGGG14450.068.62822