FastQCFastQC Report
Mon 27 Feb 2023
SRR3128256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658287
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223031.344942099889826No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG45390.27371619026139626No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG44670.2693743604092657No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC44560.268711025292968No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28020.16896954507874692No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25680.15485859805932264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74950.086.527161
TAGCGGG7000.077.968072
CGTAGGG5400.074.931012
ATAGCGG2900.074.650851
AGGGATC15550.074.349285
GGTAAGG9650.074.129521
GTAGGGC6600.073.426053
AGTAGGG27550.073.264322
TAAGGGA15550.072.314333
GACCGAT850.071.878088
AGGGCAT13800.071.8580555
AGGGATG24900.071.5339055
TAGGGTA5800.071.385754
AAGGGAT28500.071.152364
TAGGGCG3450.070.91554
AGGGTAC7700.070.8009955
TCCGCTA6400.070.593731
TAGAGGG27450.070.27462
TAGACGG1550.069.834661
AGTAAGG10000.069.652481
Produced by FastQC (version 0.11.5)